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I'm doing DNA extraction and purification for pyrosequencing. I did gel electrophoresis extraction by low-temp-melting agarose gel and cut the DNA and put into tube then used Quaigen kit for purification. Then I checked DNA recovery rate, it was around 35%. Then I checked DNA size and any contamination by Bioanalyzer 2100, but it showed very interesting image.
Before purification, my sample was around 600 bp, but after purification it increased over 700 bp. Initially I thought I made mistake or did something wrong. Then I did purification twice. But all results are same as previous one. Before purification everything is ok, but after purification, DNA size increased. I don't understand what is going on.
Can anyone tell me why and is it Ok or not?
Screen Shot 2013-02-27 at 11.31.45 PM.png 26.59K
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