NickSarbiscuit

Hi, this is my first post!

I've been working in a molecular/cellular neuroscience lab for the past couple months working with hiPSCs from patients with autism.

Everything has been fine so far but we have met with something a bit odd in the last week. Normally the media we use is quite pink (or red depending on the batch), and after a few days of being with the cultures it starts to turn more yellow, we change it every 3/4 days. However a fair amount of the media has been staying quite pink in the last week. We have some glia which have been in the same media for over a week now (they aren't needed and we wanted to see if it would eventually change) and are still pink.

It's not the batch, as we've used the same batch on other samples and it's gone yellow. We are wondering if it could be the incubator? We just autoclaved it to get rid of a fungal infection and have recently received a low CO2 reading

We feed our neurons/progenitors/glia with DMEM+Glucose+penstrep (solution = 2 mls penstrep, 0.32g Glucose, 20mls horse serum and then made up to 200ml with DMEM).

Has anyone got any ideas?

Thanks!

Nick.