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The primer in question is standard MGMT promoter region primers for methylated and unmethylated regions:
1
MGMT (MSP) unmeth F
TTTGTGTTTTGATGTTTGTAGGTTTTT
2
MGMT (MSP) unmeth R
AACTCCACACTCTTCCAAAAACAAAACA
3
MGMT (MSP) meth F
TTTCGACGTTCGTAGGTTTTCGC
4
MGMT (MSP) meth R
GCACTCTTCCGAAAACGAAACG
I am unable to find these sequences within the gene promoter region, the genbank id of which is X61657.
It be very helpful if someone could help me out with it.
Thanking you
Jeru
Ph.D Scholar,
Dept. of Human Genetics,
NIMHANS
