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Multiplexion

Member Since 22 Feb 2013
Offline Last Active Feb 25 2013 08:51 AM

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Multiplexion GmbH is a limited liability corporation and was founded as a spin-off of the German Cancer Research Center (DKFZ) Core Facility "Contamination Control" in Heidelberg, Germany, in 2012. Multiplexion is engaged in the multiplex detection of biological analytes in cell culture systems used in biomedical research. It is our goal to enable researchers at universities, institutes or industry to get safe and consistent research results.

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Website URL http://www.multiplexion.de

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bioforum
22 Feb 2013 - 16:48
Tabaluga
22 Feb 2013 - 14:26

Posts I've Made

In Topic: Cell culture contamination

22 February 2013 - 01:52 PM

shamshu27, on 12 November 2012 - 05:42 AM, said:

Hi Xuguiha,

I am facing the exact same problem ans we are unable to figure out whether these are serum precipitates or contaminants. We tested whether these could be yeast contamination but these appeared to be far too smaller than yeast cells. Since the media isn't changing color we ruled out the possibility of bacterial contamination. In addition, we have observed that in the presence of these particles my cells change morphology and also have a slower growth rate. Please inform us too in case you find a solution to this problem.

Thanks,
Shambhavi

This is true for most bacterial contaminations except the most common one: mycoplasma!

In Topic: U87MG cells not growing well

22 February 2013 - 01:49 PM

My guess is Mycoplasma. We have a mycoplasma removal or elimination protocol available online if you want to treat your cells.
Better, discard them and start with a new batch.

In Topic: contamination of human cell lines with yeast

22 February 2013 - 01:45 PM

Definitely, especially after using Saccharomyces cerevisiae at home for backing. You can contaminate your cells even two days after having used it.

In Topic: Whole lab experiencing inconsistent PCR contamination

22 February 2013 - 01:36 PM

Sounds like low level contaminations with PCR products in either any of the reagents, aerosols or materials you are using.
You do not detect them in every reaction since the concentration is too low.

In Topic: qPCR with Genomic DNA

22 February 2013 - 01:30 PM

One way is to assume that a cell contains 6.6 pg DNA. With this info you can simply translate the DNA concentration in "cell equivalents" per µL.
Next you can adjust your DNA to e.g. 1000 cell equivalents per µL and perform a tenfold dilution series. This dilution series is applied to your PCR in order to determine the detection limit of your assay.

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