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  2. → Viewing Profile: Posts: trangtrang

trangtrang

Member Since 21 Feb 2013
Offline Last Active May 17 2013 09:20 AM
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Posts I've Made

In Topic: IgG control Immunofluorescence : have problem

22 February 2013 - 02:15 PM

http://www.rndsystem...ducts/ab-105-c/
Here the ref of this IgG.
Honestly, I dont know why they use this word "normal".

In Topic: IgG control Immunofluorescence : have problem

22 February 2013 - 02:09 PM

Yes it is normal rabbit IgG .
sorry, for my bad english. But I don't understand what you means. Can you explain by different way, please?

Thanks.

In Topic: IgG control Immunofluorescence : have problem

21 February 2013 - 01:25 PM

my protocol is:
- fixation: PFA 4%
- blocking buffer: saponin/BSA/FBS; 1h/Room temparature
- primary antibody: polyclonal rabbit or IgG; OVN/4°C
- secondary antibody: rabbit; 1h/room temparature
- mounting with Prolong gold antifade.

actually, I took the IgG from other in my lab. She used this IgG for negative control in CoIP. I tried this IgG 'cause I just want to check my staining of primary antibody is specific or not.
IgG is from R&D Systems.

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