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Peniel

Member Since 20 Feb 2013
Offline Last Active Yesterday, 05:25 AM

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Topics I've Started

Any tips on how to avoid lifting cells in 96well plates

20 April 2013 - 03:59 AM

Hi everyone,

I work with MTS assay for drug inhibition. I did a practise run before the real experiment and tried dosing the drug each day. Whilst changing the media I realized that some of my adherent cells tear off and form a scaffold and some get lost. A549 seems fine and does not get dislodged but some other cancer cell lines do. Has anyone experienced this and would like to give some tips? I'd appreciate it.

Thanks

Calculating Drug concentrations in Log scales

03 April 2013 - 02:14 AM

Dear all,

I worked out how to carry out my MTS assay drug concentrations as 10uM, 20uM, 30uM, 40uM, 50uM, 60uM, 80uM and 100uM. I was just told it is better to work in log scales for drug concentrations and I have no clue on what to do. I am just confused. Please help!!!

Drug concentration

04 March 2013 - 07:18 AM

This question might seems stupid but please bear with me I'm quite new at this. I want to use a cytotoxic drug on some cancer cell line. The drug is available in 10mg and 50mg. I need to dose this cell lines with 75mg, 150mg, 300mg, 600mg, 1000mg and 1200mg each day. the molecular mass of the drug is 236.29. How do I work it out. Will I have to buy many of this compound?

Thanks for your help

Freezing DMSO

27 February 2013 - 06:34 AM

Is it ok to freeze 1%DMSO and 0.01%DMSO for future use in MTS assay as drug carrier? I know people it at 10% not sure if lower concentration makes a difference.

Which is the best way to generate growth curves for cancer cell lines

25 February 2013 - 02:55 AM

Hi all

I am quite new in all these processes and would love some ideas.

I want to generate a growth curves for lung cancer cell lines. They are adhering cells. Do I have to always trypsinize them every 12hrs and count them using a haemocytometer?
or
Do I seed them on 96 well plates and have different plates for 12hrs. 24hrs. 48hrs. 72hrs and so on and then add MTS reagent to read off the absorbance. I am worried about this though because MTS does not stop the growth so they might increase within the 3hr incubation period. I want to be as accurate as possible.

Thanks you all for your kind response.

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