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Hi,
I am starting to use hprt mutation assay and am therefore going through various protocols. One thing I am not sure of and am hoping that some of you might be able to clarify this to me. The S9 treatment, is it necessary to make additional sample with S9 treatment (for metabolic activation)? I will be using ENU (Ethyl-nitrosourea), which does not need metabolic activation, in my assay and am wondering that do I need to make additional sample with metabolic activation? Frankly, I am a bit puzzled about this whole metabolic activation thing... Please, could somebody help me?
Cheers,
J
