Find content
Not Telling
hello,
No, I did not strip the membrane. The size of protein is 10KDa. I am using 5% milk for both primary and secondary antb.
I did not increased the exposure time. I dont know if POD ECL substrate is still active after 10 min (this is the recomandation of roti lumin). The membrane was with de right face up and the antibody is ok because it work in immunohistochemistry. The substrate should be ok also.
But it is possible to did not see the background?
I din not add the primary antibody together because they are both rabbit. But this is a good ideea for future.
I will ty to use other antibody today, to see if here is signal or not and I will be back with result.
Thank you for your answer it is very useful for me.
Ana
