We have some genes that we want to test at higher levels of expression. Apart from knock-in, does anyone have any idea how to do this? SINEUP would be one way, but its quite a new technology so I was wondering if anyone has given it a try?
I can use siRNA to knock down and validate the effects of genes that are down regulated. How do you validate genes that are up-regulated? I have been thinking of using SINEUP which can increase expression of targeted endogenous genes. Does anyone have any experience with this or tried alternatives?
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