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  2. → Viewing Profile: Posts: neoplazi

neoplazi

Member Since 11 Feb 2013
Offline Last Active Feb 21 2013 07:07 AM
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Posts I've Made

In Topic: Successful Transformation->Getting Colongy--> Getting DNA after miniprep

15 February 2013 - 07:26 AM

After your suggestion also I want to sequence it. But unfortunately my supervieser has stuck mentality and forcing me for extra repeat.

Good bless me Posted Image

In Topic: Successful Transformation->Getting Colongy--> Getting DNA after miniprep

13 February 2013 - 06:22 AM

Dear Phage and Bob,

Thanks for your interest.
You are kind and helpful.

After your posts I digested main (stock) plasmid with other transformed colony miniprep results.
Here my gel image:

I was finding extra and incorrect band (end of hole) and I saw it in stock plasmid.
Do I have plasmid contamination?

I do not have sequence posibility for now.
Can you offer another suggestion?

In Topic: Successful Transformation->Getting Colongy--> Getting DNA after miniprep

11 February 2013 - 10:22 PM

But the plasmid is not new!

We took from different group that has been worked long time.

So there is no problem with construction of plasmid.

In Topic: Successful Transformation->Getting Colongy--> Getting DNA after miniprep

11 February 2013 - 08:48 AM

I have the plasmid map.

So I am checking bands from it.

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