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marian18

Member Since 11 Feb 2013
Offline Last Active Feb 12 2013 03:53 PM
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Posts I've Made

In Topic: Calibrator sample

12 February 2013 - 03:57 PM

Hi

Thanks for your replies.

I am trying to use the relative quantification method (not absolute). Hence, I believe it is not necessary for me to create a plasmid.
Therefore my question is, how to calculate fold change in gene expression if your gene is not expressed in your calibrator sample? I think I may just have to choose another calibrator sample and calculate the fold changes, and merely note that my undifferentiated cells did not express my gene of interest.

Also does anyone know how to calculate delta standard deviations for fold changes?

Thanks

In Topic: Calibrator sample

11 February 2013 - 02:44 PM

Yes, two separate primers were used. I do have a positive control to show that my GOI is working. Moreover, I do see an increase in expression of this gene at the other time points. However what I want to show from my results is, that compared to the undifferentiated cells (calibrator sample), the levels of gene expression increase over time.

Do you know if this is possible?

Thanks

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