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ahmadfathi

Member Since 06 Feb 2013
Offline Last Active Apr 29 2013 05:33 PM
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Posts I've Made

In Topic: Co-transformation

26 March 2013 - 10:45 PM

thank you for your opinion, the purpose of co-transformation is to insert another plasmid into a host which is will help my vector-insert to be expressed well. Right now, i already got it by performing standard transformation with combination of two plasmid in less than 10% of competent cell volume..thank you very much

In Topic: PCR and restriction enzyme digestion

17 February 2013 - 12:25 AM

do you means i still need redo the transformation,or just optimize the extraction for restriction enzymes digestion?some of my colleague said these happen because of insufficient concentration of DNA that need for the digestion..

In Topic: DNA ligation and trasformation

11 February 2013 - 07:22 PM

tq..
littlepumpkin:

View PostLisa Hsu, on 08 February 2013 - 03:53 AM, said:

Is it a high copy number vector ?
Your ligation sounds fine to me Posted Image
Is it because your insert DNA too toxic for the cell because of the high copy number vector?
It's just a thought Posted Image
what you mean by toxic?
i really need a clearance, i'm still a newbie in this area
my insert size is 1.5k bp..

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