Hi folks. I am doing a cytotoxicity assay on human lymphocytes. I am using two different test substances (of my choosing) and I am going to run my cells in a 96 well plate through a plate reader. This program will give me absorbacne values that i am supposed to put into excel and generate a graph. How do I get a lethal dose from there? Any help would be appreciated. Also, am I plotting absorbacne with time or some other parameter/variable?
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