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Jessica Crowley

Member Since 05 Feb 2013
Offline Last Active Feb 11 2013 10:04 AM
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Whole lab experiencing inconsistent PCR contamination

05 February 2013 - 09:33 AM

Our lab is dealing with some very pesky contamination in our negative (water only) controls lately. It seems in some of our assays we are seeing the wildtype band, the transgenic band, or even both. The most confusing part is the inconsistency of it all. We can run three assays together, each with different primer sets and only one will show the contamination. That means the only thing changing is the primers, but we will have used the same primers in the past with no problems. Unless we have all suddenly forgotten how to do PCR, there is something fishy going on. Notes:
  • dNTPs have worked fine for other Assays and QPCR
  • We cleaned our pipets and always use barrier tips
Can anyone offer any advice? If we could at least pinpoint where it is coming from, that would help. Thanks!

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