Right now we are to the point where when it deosn't work we just have to re-do it and hope for the best. Everytime it doesn't work we just replace the primers just in case, but I just don't think it's that. I honestly am starting to think it's our water aliquots. We just got these new colorful 1.5 ml tubes and they don't really close all the way. I think that is a link and I'm just going to blame the ungergrads for now too I think.
Sometimes we see only wildtype, but sometimes both the wt and transgenic band. It usually shows up in the water negative controll and all the samples end up having the same genotype.
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