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It is very hard to check DNA quality after bisulfite treatment because the DNA is tiny in amount and has been degraded considerably. Measuring concentration on Nanodrop won't give you much information. If you get high reading on 230nm, it could be that the sample has high salt concentration. Because for bisulfite PCR, many factors not just template could contribute to PCR failure such as primer design, PCR conditions, you need to troubleshoot all possible causes. Can you post your PCR conditions here?
#149567 Quality of Bisulfite Converted DNA
Posted
on 04 February 2013 - 10:12 AM
