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The calculation for molar ratios is:
insert ng = ratio x [insert length /vector length] x vector ng
where the ratio is a number, e.g. for a 3:1 you would use "3".
Insert and vector is length in bp.
#151262 Problem with ligation using pJET and pGEM for clone library
Posted
on 28 February 2013 - 12:06 PM
