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Hi,
I am experiencing problems when culturing primary monocytes. After I use LPS to stimulate them and asses for differences in gene expression between stimulated cells and mock, changes are either similar or in mock even bigger. I decided to rest the cells for 24 hours first and do stimulation after this time, but no change. So, I think there may be endotoxins in serum I use (RPMI 1640 + 10% FBS - Gibco). However, I am not able to find any producer who offers endotoxin-free or at least endotoxin-low serum. Does anyone of you have any suggestion?
Thanks a lot!
Paja
