I want to amplify and clone my gene of interest along with its native promoter. My question is how many base pairs should be included to constitute the promoter. A lot of papers I read include 1500 or 2000 bp upstream. However, I noticed for my gene there is another gene located immediately upstream. From the stop codon of the upstream gene to the start codon of my gene of interest is only 303 bp. If the 3'UTR of the upstream gene is considered, there is only 92 bp between them. So how much should I include if I want the promoter? 303 bp? Could I just include 1500 or even 2000 bp even though it is part of another gene? Thanks for the help.
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