I really need help! I am new to running ELISA plates and I have run them multiple times for the past few weeks but I am still not getting the results I need. I am noticing variations between my duplicates. Recently I had my supervisor run a plate along side me and he had issues with his plate in the same places I had issues. He did point out a pipetting error on my behalf but other than that he said I did everything right, he also checked all of my calculations which were also correct. Does anyone have any suggestions about what could be going wrong, I just find it alarming that he is so experienced with running ELISA plates and his had issues too. :-(
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