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Not Telling
I agree that non-functional IFN could explain both results. Try with a fresh batch and do a western blot in parallel to be certain that the IFN is working. The phospho-Stat1 antibody you are using is absolutely amazing. I've used it for IF and flow cytometry ant the signal to noise is wonderful.
#149103 Need help on STAT1-GFP nuclear translocation
Posted
on 29 January 2013 - 01:11 AM
