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youngpadawan

Member Since 25 Jan 2013
Offline Last Active Feb 01 2013 06:16 PM

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Genomics

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pito
25 Jan 2013 - 05:03

Topics I've Started

Career Path with Masters in Genetics??

25 January 2013 - 02:27 AM

Hello,

I going to graduate with a degree by this May and I realised I really love genetics. I would really love to learn and understand more about this field. But my problem is that genetics is a wide field, I dont know which to choose specifically for further studies. I dont prefer microbiology. My questions are - Can you tell me the areas of genetics? what is the best masters degree in genetics? Which is better, coursework based or research based masters degree? What can I do with a masters degree in genetics besides being involved in academics?

How To Culture MRC-5 cell line

25 January 2013 - 02:18 AM

Hello there. Im trying to culture the MRC-5 cell line. Im using DMEM + 10% FBS medium. After 5 days, the confluency only increased from 15% - 50% and there are still some cells floating. My questions are:

1. Why is this happening and how do I prevent it?
2. How long does it normaly takes for the number of MRC-5 cells to double?
3. What is the best protocol to detach the cells from the flask without damaging the cells?