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hi,
i made a construct of the human TSHR with a mcherry fluorescent tag at its C terminus, for my internalization and tracking experiments with microscopy. however, what i have seen is that the processed protein is found to be in endosomes and also on the cell surface (where it should be) when expressed in HEK cells. i cant understand the reason why this tagged TSHR appears to be in endosomes. is it because of this tag ??? because a similar construct, but with a YFP or GFP tag functions perfectly, and the TSHR is found only on the cell surface.
is the presence of the tag, viz a red fluorescent protein, confers some properties on the localization of the TSHR, a GPCR. ???
