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nvramakanth

Member Since 22 Jan 2013
Offline Last Active Feb 04 2013 05:54 AM
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#148579 I have a 96 tandem array repeat clone but need half of them in new clone

Posted bob1 on 22 January 2013 - 11:31 AM

Two ways-
1) find a cut site in the array that will cut it in half more or less, blunt the cut site and do something similar for the other end of the plasmid.
2) PCR the array off the plasmid using primers with unique cutting site incorporated.


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