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PhDinAcronyms, on 03 February 2013 - 07:49 PM, said:
Maybe trying a lower percentage gel would make transfer easier as 18% is fairly high.
I totally agree! 18% is way too thick... For detecting a 18kD protein I usually use 15% polyacrylamide gel with the same transfer condition (100V, 60min, 4oC) with no apparent problem!
Good luck!
