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I am writting to ask whether anybody had a same problem as mine. My protein samples are floating out of the Tricine-SDS-PAGE. I prepared the gel according to Schagger's Nature Protocol. I checked pH of every buffer (anode, cathode, gel buffer). My protein samples were dried well and resuspended in reducing buffer (the same as in the publication), so I think the influence of traces of alcohol can be omitted. Even ready-to-use protein marker floated out. Does anybody know what could cause such a problem?
Thank you in advance,
Kasia Roeske
