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EwaKaniewska

Member Since 15 Jan 2013
Offline Last Active Jan 28 2013 02:33 AM

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London/Gdansk

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primary cells transfection

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cellgene
15 Jan 2013 - 06:10

Topics I've Started

primary valve interstitial cells transfection

15 January 2013 - 02:20 AM

Hi everyone!

I want to do transient transfection of valve interstitial cells isolated from pig aortic valves. I can't use viral vector, and at first I really need to try transfection by some reagent (after that I'll have opportunity to do this by electroporation). Can you suggest me any thing? I've try to use PEI but I can't see any effect (EGFP vector). Can you suggest anything?

Thanks!
Ewa