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In Topic: minimum recombinant protein amounts for gst pulldown
15 January 2013 - 05:39 AM
I can sent you my protocol if you want
In Topic: minimum recombinant protein amounts for gst pulldown
15 January 2013 - 02:40 AM
Hi,
during my master I've been using 1-2 ug protein (purified by me) with GST tag for pull-down. But I've been using 20 ul Glutathione Sepharose not ready kit for co-percipitation... I don't know how it will be work with your protein (you need to check conditions ), but in my opinion you can try.
Ewa
during my master I've been using 1-2 ug protein (purified by me) with GST tag for pull-down. But I've been using 20 ul Glutathione Sepharose not ready kit for co-percipitation... I don't know how it will be work with your protein (you need to check conditions ), but in my opinion you can try
.Ewa
