Blindspot

I hope i chose the right sub for this question.

I have been doing the same protein purification for the past few months, only with the murine for of the protein.

After eluting the protein from my Ni-NTA column (whole process at 4C in the cold room) using a Tris-HCl (pH 8) and imidazole-based buffer in 10ml fractions, i put the fractions on ice and left them in the cold room for the past two days.

Today i wanted to continue and see that the fractions where i predicted the protein to be had big pellets.

Is this reversible? How? I need to dialyse the stuff for the next two days.

What i did right now was dilute the fractions (added 5ml of elution buffer) and left them to shake at room temperature.