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Blindspot

Member Since 14 Jan 2013
Offline Last Active Jan 15 2013 08:32 AM
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Posts I've Made

In Topic: Protein precipitated while kept at 4C over night after elution from a Ni-NTA col

15 January 2013 - 02:03 AM

I put them through the first dialysis over night (also in the cold room) and the precipitate is still there, but it looks "different".
Do you think i should do the dialysis at room temperature? It seemed to improve the solubility, but this might just be some eye-bias.

If i do that, i am afraid to destroy my protein...

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