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For my 2 cents I think it's very important that you use a sensitive luminometer setup and this is probably why Lonza have produced a large table of recommended machines for the assay (http://bio.lonza.com...uminometers.pdf).
Using a BMG Pherastar with a luminometer optic module, I regularly find my negative and positve controls work as expected in the assay. My only concern is that there is a high false positive rate and that's going to take me some time to determine this value.
The samples that were identified as mycoplasma positive in this assay turned out to be negative by PCR and Hoechst dye test.
Sample Luminescence (A) Luminescence (
B/A.Blank 3924 332 0.08
Medium (DMEM) 1129 376 0.33
Cell sample#1 2318 27849 12.01
Cell sample#2 2839 10070 3.55
Positive Control 3503 31388 8.96
