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Toastayy

Member Since 09 Jan 2013
Offline Last Active Jan 25 2013 09:20 AM
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Posts I've Made

In Topic: Problems with growing out transformants in liquid LB

09 January 2013 - 03:49 PM

Well the lawn was only seen on 1 of the plates. There were sparodic clusters of colonies on various portions of the plate.

The Amp conc. is at 100microg/microL.  I remade new plates and will get the results tomorrow so i'll update this with those results.

Also, The controls seemed okay: 1. No colonies on cells only or vector only ligation.  And colonies were seen on an uncut plasmid at 1:1k dilution. However, skepticism tells me that some of the colonies on that plate were also satellites.

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