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Hello, I've been doing some BIS modification using the Genetic Signature MethylEasy Kit. I got bands of the correct size with my modified primers and immediately ligated the products and procede with cloning in pGEMTeasty. My product is 1626bp in length. I've sent 10 clones for sequencing and the sequences that I have received show most of the cytosine residues to be methylated excepted those in the first ~250bp which are non-methylated and have been converted to Ts. Is this possible or is it more likely that the treatment has failed?
Thanks in advance!
