Javascript Disabled Detected

You currently have javascript disabled. Several functions may not work. Please re-enable javascript to access full functionality.

wanzybio

Member Since 06 Jan 2013
Offline Last Active Feb 07 2013 10:41 PM

Find content

Posts I've Made

In Topic: ELISA, BDNF, Biotinylated Conjugate

28 January 2013 - 08:31 PM

Not yet started working on human blood. I have been trying to figure out the combinations of Antibodies that works for my rest of the project on human blood. Presently, I have been doing experiments on rhBDNF protein to find the best Ab pair combination.

In Topic: ELISA, BDNF, Biotinylated Conjugate

23 January 2013 - 05:15 PM

Need to talk to my supervisor. I can't do it myself. Will let you know. Thanks a lot for sharing the information.

In Topic: SDS PAGE gels

22 January 2013 - 08:25 PM

Instead of going O/N incubation you can transfer for 1 hr at 40v,170mA.

In Topic: Biotinylated BDNF

22 January 2013 - 08:13 PM

Capture is msMab#9 or (BDNF#9) at 3ug/ml, detection is Biotinylated msMab#1 or (BDNF#1) at 2ug/ml. I am following kit provided by Biosensis Pty but not the antibodies provided in the kit. My blocking buffer is Superblock Blocking Buffer in PBS (ThermoScientific).I dilute the Streptavidin-HRP in a sample diluent buffer at 1:100 dilutions and add 100ul per wells. My incubation time is O/N for capture, blocking - 1 hr, Antigen(sample) - 90 mins at RT in a shaker(100RPM), detection - 30 mins at RT, 100 RPM and HRP - 1 hr at RT, 100RPM. I hope this could help you. Thank you.

In Topic: ELISA, BDNF, Biotinylated Conjugate

22 January 2013 - 08:02 PM

Thank you very much. My blocking incubation time was 2 hrs at RT in a shaker (100RPM). I tried it with O/N incubating. My capture Ab incubation time is O/N at 4^oC. For antigen(sample) its 90 mins, detection is 1 hr and HRP is 1 hr at RT in a shaker (100RPM). I didn't have any idea about NaCl. I will increase the NaCl concentration and perform it. And yeah the world of science is really confusing, once you are into it, you keep on loving... Posted Image

Once again thank you very much for the information.

Google Sign in options

wanzybio

Community Stats

About me

Contact Information

User Tools

Friends