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Pangea

Member Since 03 Jan 2013
Offline Last Active May 14 2013 03:22 AM
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#147962 pET 32 (+)

Posted Pangea on 13 January 2013 - 09:38 AM

What means the "+" in the vector description? What kind of orientation is it?


#147946 Suggestion for a book!

Posted Pangea on 13 January 2013 - 06:49 AM

Listen type into google search as follows: Cytokine filetype:pdf  

The part filetype will give you just PDF files and gives you a smile in your face:). You can do this also with doc, xls, etc. Enjoy the discovery.


Books:
Cytokine Protocols (Methods in Molecular Biology)

http://elib.fk.uwks....ogy Series].pdf

http://www.ctf.edu.t...1_Cytokines.pdf


#147945 How to raise polyclonal antibodies against whole bacteria live &/ or inactiv

Posted Pangea on 13 January 2013 - 06:41 AM

Thats just general information, but which bacteria you want to produce rabbit Ab. It will be take 3 month i guess in rabbit. Whole bacteria means just its capsule, isnt? You might look up the consistence of the capsule. It dosent matter if it Gram-negative or -positive, isnt? But i guess it will be unspecific Antibody. Do your Bacteria has specific marker molecules on his surface?

http://www.uniprot.org/keywords/875

http://ecvam.jrc.ec....hopReport35.pdf


#147895 Open-Source Mathematics in Research

Posted Pangea on 12 January 2013 - 07:35 AM

Do someone has a good source for general mathematics in the Lab?


#147472 DNA extraction - PCR Problem

Posted Pangea on 04 January 2013 - 12:52 PM

You must have a isolation protocol, as far i know if you have good primers ploidity should not be a problem. You are not the first person working with. It tells you just the copy number of identical chromosomes. Isolated DNA should be clean.


#147390 GST- tagged protein expressed in insoluble

Posted Pangea on 03 January 2013 - 10:46 AM

SDS-Page check. İn Frame check.


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