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Some adherent cells grow in suspension when you use serum-free media. However, be aware that this can change the characteristics of the cells ! I also know that sigma at least has a serum-free medium for CHO suspension cells, but I think it's expensive and probably other serum-free media are also going to do the trick.
#147547 How to transform adherent cells to suspension (CHO cells)
Posted
on 05 January 2013 - 03:13 PM
#147516 Ethidiumbromide
Posted
on 05 January 2013 - 07:22 AM
SYBR Green for example..
You should be able to find them yourself....
However keep in mind that some of the alternatives seem to be more "dangerous" then ethidium bromide itself.
PS. the horror stories about ethidiumbromide are really over the top and not true at all. Many people seem not to be able to search for information themself and just accept "stories" others told them.
You should be able to find them yourself....
However keep in mind that some of the alternatives seem to be more "dangerous" then ethidium bromide itself.
PS. the horror stories about ethidiumbromide are really over the top and not true at all. Many people seem not to be able to search for information themself and just accept "stories" others told them.
#147506 Questions about proper technical approach
Posted
on 05 January 2013 - 02:52 AM
Hi Mindologist,
I'll go straight to your first question. The first thing that came to my mind is to do what's called the genome walking. Bear in mind that I have not done this (or anything similar) before so someone here might have better alternatives.
Here are some stuffs that could be useful:
www.clontech.com/xxclt_ibcGetAttachment.jsp?cItemId=17326
http://www.protocol-...osts/37995.html
www.ucl.ac.uk/~ucbhjow/b200/Cloning_genomic_DNA.doc
http://www.biotechni...ques-44287.html
https://www.ncbi.nlm...cles/PMC306810/
I'll go straight to your first question. The first thing that came to my mind is to do what's called the genome walking. Bear in mind that I have not done this (or anything similar) before so someone here might have better alternatives.
Here are some stuffs that could be useful:
www.clontech.com/xxclt_ibcGetAttachment.jsp?cItemId=17326
http://www.protocol-...osts/37995.html
www.ucl.ac.uk/~ucbhjow/b200/Cloning_genomic_DNA.doc
http://www.biotechni...ques-44287.html
https://www.ncbi.nlm...cles/PMC306810/
#147483 siRNA in E.Coli
Posted
on 04 January 2013 - 03:50 PM
Nope. But bacteria have a similar mechanism called CRISPRs (C lustered R egularly I nterspaced S hort P alindromic R epeats).which confers resistance to exogenous genetic elements such as plasmids and phages. This mechanism also uses RNA as guide, but use proteins other than AGO as in eukyaroytes.
