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JYaron

Member Since 02 Jan 2013
Offline Last Active May 14 2013 02:06 PM

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Community Stats

Group Active Members
Active Posts 13
Profile Views 299
Member Title PhD Candidate, Biological Design
Age 25 years old
Birthday March 31, 1988
Gender
Male
Location
Tempe, AZ

About me

Real Name
Jordan Yaron
Lab or Group
Center for Biosignatures Discovery Automation
Institution
ASU Biodesign Institute
My research interests
Inflammation, Caspase-1, Pyroptosis, Cell Biology, Immunology, Single Cell Analyses

Contact Information

Website URL http://www.jordanyaron.com/

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ricdav
21 May 2013 - 12:20
pito
13 May 2013 - 23:18

Topics I've Started

Protocol for detecting soluble, secreted proteins on a Western?

13 May 2013 - 05:55 PM

Does anybody have a good protocol for detecting soluble, secreted proteins on a Western after resolving by SDS-PAGE?

I want to detect interleukin 1-beta produced and processed by macrophages upon stimulation of the NLRP3 inflammasome.

Do I have to run the supernatant through a molecular weight cutoff filter in order to concentrate it? Or can I just add loading dye to the supernatant and run the gel?

Thanks!