Does anybody have a good protocol for detecting soluble, secreted proteins on a Western after resolving by SDS-PAGE?
I want to detect interleukin 1-beta produced and processed by macrophages upon stimulation of the NLRP3 inflammasome.
Do I have to run the supernatant through a molecular weight cutoff filter in order to concentrate it? Or can I just add loading dye to the supernatant and run the gel?
JYaronMember Since 02 Jan 2013
Offline Last Active May 14 2013 02:06 PM
- Group Active Members
- Active Posts 13
- Profile Views 299
- Member Title PhD Candidate, Biological Design
- Age 25 years old
- Birthday March 31, 1988
Lab or Group
Center for Biosignatures Discovery Automation
ASU Biodesign Institute
My research interests
Inflammation, Caspase-1, Pyroptosis, Cell Biology, Immunology, Single Cell Analyses
- Website URL http://www.jordanyaron.com/