Mindologist

Hi,

I just want to know what competitive transplantation experiment is, and how to proceed it, and what the competitive transplantation experiments is to see.

Thanks a lot in advance.

Helow,

I think I found an active promoter region on the human genome, and I guess a certain gene is expressed by the promoter.
Problem is I don't know how to design a series of experiment in order to the promoter that I found is leading to a gene expression.
Could you give me a clue?
Thanks a lot of your helps in advance.

Hi all enthusiast,

I just want to know the exact mechanism of retroviral genome integration into host genome.
More specifically, I want o know exact viral DNA sequences that are involved in the integration and host or viral proteins that contributes to the integration.
Any source of the information would be OK for me, because I couldn't find any inforamtion about it.
Thanks for all your help.
Have a great day.

Hi all enthusiast,

I'm looking for good advices about producing lentivirus.
My problem is that there is no RFP (reporter) cells after transfection with viral construct and packaging plasmids.
I'm using second generation packaging system, pPAX and pMDG, and my viral construct is pSIN4 vecter with a gene of my interest.
For producing virual, I'm using 293T cell, and I'm using Calcium phosphate method for transfection.
When I transfect only my viral construct (containing RFP gene), I could see RFP+ cells next day (efficiency is good).
However, after transfection with my viral construct + packaging plasmids, there's no RFP+ cells next day.
I don't know what's going on.

So, I'm looking for senior's advices and solutions.
Thanks a lot for your helps.
Happy new year!