I just saw your great comments. Thank you a lot, bob1. I'm going to try different ratio of the plasmids. Do you have any ratio that you may suggest me? If you do, it would be great for me.
Besides, other question is about serum free medium. I searched internet and saw some are suggesting using serum free medium during viral production, but others just use serum-containing medium.
Which one do you think is better?
I really appreciate to you for the good suggestion and sharing knowledge.
Thanks a lot, Pangea and Julio, for the good information and knowledge.
One question for Pangea. Since I don't know anything about bioinformatic tools, do you have your favorite one? or could you recommend one of the tools?
Thanks again.
Thank you, Pangea, for the reply.
But, now that I see my question, it's kind of wrong, and even my english is terrible. I'm really sorry about that.
My situation is like this.
I found an active promoter on the genome, and I confirm its activity by reporter assay.
Now I'm thinking that there may be a gene which is located down stream of the promoter that I found, and the gene is under the control of the promoter.
To prove my idea about a gene that may be located down stream of the promoter, what should I do? What is the best approach to identify the gene that might be there?
I have no idea what to do regarding this.
Could you give me a clue?
Thanks a lot of your helps in advance.
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