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  2. → Viewing Profile: Topics: Dai Le

Dai Le

Member Since 29 Dec 2012
Offline Last Active Mar 12 2013 03:16 AM
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Topics I've Started

Help Plz! Hes1 antibody for Immunohistochemistry

22 January 2013 - 06:47 PM

Hi everyone,
Could you guys suggest a trustworthy Hes1 antibody for immunohistochemistry on Frozen section? In my lab, we have only small amount of home-made Hes1 from Kyoto Uni, Japan. We also have Hes1 anitbodies from Abcam and Scbt but they don't work at all.
Looking forward to receiving replies
Thank you in advance

Culture of Drosophila S2R+ cells

29 December 2012 - 07:12 AM

Hi guys,
I'm new to this forum. Lately, I've been working with S2R+ cells and this thing is really pissing me off. I don't know why I cannot culture this thing. I have tried different FBS, both heat inactivated and non heat inactivated. These cells don't seem to attach to the culture surface when I cultured under Schneider + 10%FBS. When I switched to serum free media Sf900 II, they attached to the surface but didn't proliferate. Could anyone with experience in this thing give me some tips?
Thank you guys in advance

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