@Tabaluga: Well, I have searched the literature. They all use antibodies from Japanese guys. People only use commercial brands for in vitro (cell culture)exp. That's why I'm asking if you guys have any about trustworthy commercial Hes1
@Curtis: I used polyclonal antibody. It's the best for IHC. FYI, Abcam products are the best for IHC. They are very expensive. I don't know why none of the commercial Hes1 antibodies works. People always use home-made Hes1 for IHC. But you know, it's hard to ask for antibody if you are not in same group of research.
Anyway, thanks