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You must have a isolation protocol, as far i know if you have good primers ploidity should not be a problem. You are not the first person working with. It tells you just the copy number of identical chromosomes. Isolated DNA should be clean.
#147472 DNA extraction - PCR Problem
Posted
on 04 January 2013 - 12:52 PM
#147263 different PARTS different DNA ,,,
Posted
on 30 December 2012 - 12:07 AM
No, I did not mean mutated after extraction/contamination. What I mean is allelic variation in diploid or triploid chromosome set.
#147228 different PARTS different DNA ,,,
Posted
on 28 December 2012 - 06:40 PM
For straight amplification off the DNA the ploidy should not matter, in fact, the more copies there are, the easier the amplification should be.
#147211 different PARTS different DNA ,,,
Posted
on 28 December 2012 - 12:26 PM
relative sequences of DNA in diploid, triploid should be more or less conserved. As long as your primer binding site is not mutated in other chromosome set there should not be problem.
