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Hamidrad

Member Since 28 Dec 2012
Offline Last Active Jan 13 2013 01:14 PM

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Active Posts 4
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Age 25 years old
Birthday November 28, 1987
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My research interests
Food Safety, Genetically Modified Products, Dairy Industry

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pito
23 Jan 2013 - 13:17

Posts I've Made

In Topic: DNA extraction - PCR Problem

05 January 2013 - 02:23 AM

Pangea, on 04 January 2013 - 12:52 PM, said:

You must have a isolation protocol, as far i know if you have good primers ploidity should not be a problem. You are not the first person working with. It tells you just the copy number of identical chromosomes. Isolated DNA should be clean.

Thanks. But I didnt find survey on dna isolating from pulp and peel of bananas!! But there are a lot of research on dna extracting from leaf and root of it. So We should try to have good isolation and then select a suitable primers for pcr and dont focus on searching ploidity of banana's dna before dna extracting?... OK

In Topic: different PARTS different DNA ,,,

29 December 2012 - 11:55 AM

prabhubct, on 28 December 2012 - 12:26 PM, said:

relative sequences of DNA in diploid, triploid should be more or less conserved. As long as your primer binding site is not mutated in other chromosome set there should not be problem.

Thanks,
but you mean If the part which dna extracted from that, was in triploid form, may be mutated after extraction? It may occure because of contaminations?