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I know plenty of people who only add supplements (like FBS, A/A, etc.) without removing any of the base media (DMEM, a-MEM, RPMI, etc). The correct way to do it would be to proceed like you are making a buffer, starting with 60-80% volume of base media (like with water for buffers), add any supplements like 5-20% FBS, antibiotics, antimycotics, etc., then q.s. to final volume with your base media.
The difference in the concentration of serum you are using is very minor, I would not worry about it. If you want to be absolutely sure you would have to repeat your experiments with the correct concentration, of course. The important thing right now for your experiments, if you are in the middle of one, is to be consistent -- make it the exact same way each time throughout the entire experiment so if it does have an effect, it won't be a variable for analyzing your experimental results.
#147134 Quick question about making culture media.
Posted
on 26 December 2012 - 07:10 PM
