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lioiviei14

Member Since 22 Dec 2012
Offline Last Active Jan 02 2013 07:22 PM
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Topics I've Started

What is the role of SV40 sequence in "5'UTR - GFP - SV40"?

02 January 2013 - 07:25 PM

Dear,
For expression of GFP, one used 5'UTR - GFP - SV40 this sequence. What is the role of SV40 sequence in "5'UTR - GFP - SV40"?
Thank you in advance.

What is "unmasked genome(%)" ?

22 December 2012 - 10:31 PM

Dear all,

I read a paper, 'Amplification free illumina sequencing-library ...' by Kozarewa.
Figure 2 in this paper, y axis is denoted as 'unmasked genome(%)' and x axis is depth of genome base coverage.
I guess the number of certain base according to coverage follows Poisson distribution, but I don't know what the relationship with "'unmasked genome(%)" is.
Simply, 'unmask' what?

Thank you.

Can "3' - biotinylated oligo" be ligased with "5' phosphoryl

22 December 2012 - 02:36 AM

I have 3' biotinylated oligo, Can it be ligated to 5' end of oligo? On what site of DNA a biotin linker is covalently connected?

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