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Thanks for you reply,
I am not sure what you mean by to use 6-7% ? is it a gredient of 6-7% ?? But I have to study 4more proteins on the same blot which has various molecular weights.
556kDa, two proteins with 95kDA, 90kDa, 65kDa and 26kDa.
and one housekeeping protein as well!!
Do you think whitout cutting I can perform this?? I have all antibodies from cross species!! It is really complicated.
If you have any suggestion for me please do post it.
Thanks,
Preeti
