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gubi

Member Since 17 Dec 2012
Offline Last Active Jan 29 2013 06:19 AM
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Posts I've Made

In Topic: RNase inactivation during IHC prior to LCM

21 January 2013 - 06:10 AM

View PostMyworkismyplay, on 26 December 2012 - 08:06 PM, said:

I would suggest trying to do everything on ice if you are not already. What is your RNA extraction method post-IHC?
Thank you for your suggestions! I have performed the IHC on ice (or actually in the fridge w. ice cold reagents), and it doesn't help. I purify my RNA with a column kit (Arcturus PicoPure) that I know works well, and always include a control which is fine.

In Topic: RNase inactivation during IHC prior to LCM

21 January 2013 - 06:08 AM

View PostCurtis, on 23 December 2012 - 11:03 PM, said:

After fixation and permeabilization anything can penetrate the tissue.
I would have thought so too, but even the addition of 3U RNAsin/µl ab dilution ect. does not seem to preserve my RNA. So I believe I have a lot of endogene RNases that just does not get inhibited fast enough:-(

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