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Mighty Mouse, on 08 December 2009 - 06:35 PM, said:
The Qiaquick kit from Qiagen works just fine with the millipore kit. I make use of the Millipore magna-chip kit as well and have run into the same problem. I wrote out all the ingredients that come with the kit and how many I use per ChIP sample (I'm dissecting out brain tissue, so I use different amounts than Millipore recommends) and which reagents are my limiting reagents. For me it was the Cell Lysis Buffer and the Protease Inhibitor cocktail.
Like you, I tried to order reagents individually, but they don't sell them; but if you call Millipore they will give you the composition of many of their reagents (e.g, now I make my own cell lysis buffer) or buy them from elsewhere (I've been using the Halt protease/phosphatase inhibitor cocktail from Pierce; has worked well so far). I think it's quite obnoxious that they don't sell components individually given that various labs have different reagent requirements....
MM
Like you, I tried to order reagents individually, but they don't sell them; but if you call Millipore they will give you the composition of many of their reagents (e.g, now I make my own cell lysis buffer) or buy them from elsewhere (I've been using the Halt protease/phosphatase inhibitor cocktail from Pierce; has worked well so far). I think it's quite obnoxious that they don't sell components individually given that various labs have different reagent requirements....
MM
I have the same problem as you! I also used all cell lysis buffer and my kit is from Millipore. They say it for 22 assays. I think it's just for one sample with small amount. I have several samples every time so it's far not enough for me. I found there are many different cell lysis buffer and nuclear lysis buffer in web. But I do not know which one can work with Millipore Kit. I really appreciate if you can give me suggestion about making my own cell lysis buffer.
