Find content
Hello everybody,
I am having problem with neuroblastoma cell line, in particular when I seed the cells on the petri dish, most of them remain in suspension.
Looking on line I see that to overcome this problem some people use poly-l-lysine but I have no idea how to use it,
furthermore, sigma has many type of poly-lysine, could please someone tell me how to use it ? and which kind of poly-lysine is good in term of molecular weight ?
Regards
Patrizio
- BioForum
- → Viewing Profile: Topics: NDUFB11
Community Stats
- Group Active Members
- Active Posts 9
- Profile Views 307
- Member Title member
- Age Age Unknown
- Birthday Birthday Unknown
-
Gender
Not Telling
About me
-
My research interests
biochemistry
Contact Information
Topics I've Started
SH-SY5Y and Poly-L-Lysine
17 May 2013 - 05:21 PM
protein concentration and Pull down
04 January 2013 - 11:18 AM
Hello,
I have another question for you
I am going to set up a pull down assay using magnetic beads invitrogen.
In my previous experiment I performed a GST pull down experiment following a protocol inherited by my ex colleague.
Comparing this protocol, especially when I compared the concentration of proteins in the cell extract that people report on their papers, I noticed that I used a concentration of proteins which is too high ...sometime over then 6mg/ml of proteins whereas in the papers people report not more then 1-2 mg/ml.
My question is ..
Which is the criteria to use a correct amount of bait protein coupled as well as the protein concentration in the cell extract ?
thank you
-P
I have another question for you
I am going to set up a pull down assay using magnetic beads invitrogen.
In my previous experiment I performed a GST pull down experiment following a protocol inherited by my ex colleague.
Comparing this protocol, especially when I compared the concentration of proteins in the cell extract that people report on their papers, I noticed that I used a concentration of proteins which is too high ...sometime over then 6mg/ml of proteins whereas in the papers people report not more then 1-2 mg/ml.
My question is ..
Which is the criteria to use a correct amount of bait protein coupled as well as the protein concentration in the cell extract ?
thank you
-P
Mass Spec software
27 December 2012 - 10:48 PM
Hello guys
I would like to know if there is a free software similar to "X calibur thermo" to analyze
raw files. I have a Mac Os computer and if I could work on my desk it would be great .
hope you are having a good Christmas time.
I look forward to hear from you
-P
I would like to know if there is a free software similar to "X calibur thermo" to analyze
raw files. I have a Mac Os computer and if I could work on my desk it would be great .
hope you are having a good Christmas time.
I look forward to hear from you
-P
dialyse of a protein
11 December 2012 - 11:38 PM
Hello, I am a new member even though I know this this forum since long time ...
I have a question, I am trying to purify a protein of 11-12 Kda.
Using NTA-Ni Qiagen I have a lot of contaminant.
I know I could use other things for a better purification such as ionic exchange chromatography or something else( currently I don't have ) and I was thinking something else today, my idea is this:
my protein is 11-12 KDa, if I use the spin tube for Dialysis with a MWCO 30 KDa , do you guys think that I could collect my protein and keep the unspecific in the filter ?
And should I use a filter with a MWCO 10 or 30 ?
thank you
I have a question, I am trying to purify a protein of 11-12 Kda.
Using NTA-Ni Qiagen I have a lot of contaminant.
I know I could use other things for a better purification such as ionic exchange chromatography or something else( currently I don't have ) and I was thinking something else today, my idea is this:
my protein is 11-12 KDa, if I use the spin tube for Dialysis with a MWCO 30 KDa , do you guys think that I could collect my protein and keep the unspecific in the filter ?
And should I use a filter with a MWCO 10 or 30 ?
thank you
- BioForum
- → Viewing Profile: Topics: NDUFB11
- Privacy Policy
