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Hi guys,
I am new to immunofluorescence and I am about to try to visualize a protein in my bone marrow derived macrophages. The problem is that the secondary antibody Alexa fluor that i ordered has been delayed and I have my cells ready on my coverslips but not all the reagents. My question is can I start the IF procedure and stop somewhere before the 2ndary?? I know that for tissue you are able to leave them in Ethanol for a while after PFA fixation but I have no idea for cells... Thanks a lot for your help, LN
